Difference between Microscopic and PCR Examination Result for Malaria Diagnosis and Treatment Evaluation in Sumba Barat Daya, Indonesia

Dwita Anastasia Deo, Elizabeth Henny Herningtyas, Umi Solekhah Intansari, Taufik Mulya Perdana, Elsa Herdiana Murhandarwati*,  and Marsetyawan HNE. Soesatyo

ABSTRACT

Microscopic examination is the backbone of malaria diagnosis and treatment evaluation in Indonesia. This test has limited ability to detect malaria at low parasite density. Inversely, nested
polymerase chain reaction (PCR) can detect parasites at a density below the microscopic examina-tion’s detection limit. The objective of this study is to compare microscopic and PCR results when
being used to identify malaria in suspected patients and patients who underwent dihydroartemisinin–piperaquine (DHP) therapy in the last 3–8 weeks with or without symptoms in Sumba Barat Daya, Nusa Tenggara Timur, Indonesia. Recruitment was conducted between April 2019 and February 2020. Blood samples were then taken for microscopic and PCR examinations. Participants
(n = 409) were divided into three groups: suspected malaria (42.5%), post-DHP therapy with fever (4.9%), and post-DHP therapy without fever (52.6%). Microscopic examination found five cases of P. falciparum + P. vivax infection, while PCR found 346 cases. All microscopic examinations turned negative in the post-DHP-therapy group. Conversely, PCR result from the same group yielded 29 negative results. Overall, our study showed that microscopic examination and PCR generated different results in detecting Plasmodium species, especially in patients with mixed infection and in patients who recently underwent DHP therapy.

Keywords: subclinical malaria; asymptomatic malaria; high endemicity; nested PCR; microscopic examination